From Columbia research:
In summary, we developed HP-LAMP, which enables rapid detection of SARS-CoV-2 directly from saliva in 30 min using a simple one-step protocol with a LoD of 2 viral copies per L of saliva and a sensitivity and specificity of 97% and 100%, respectively. Performing the assay requires only a heat block and a 1.5 mL microcentrifuge tube prefilled with a mixture containing the necessary enzymes, primers, buffers and reagents to simultaneously perform reverse transcription and amplification of the SARS-CoV- 2 viral RNA, while blocking the naturally occurring inhibitors and nucleases found in saliva. This simple method can be easily scaled and deployed to centralized laboratories, points-of-care and field locations where testing is greatly needed.
Acknowledgement
We thank Drs. Kevin Roth, Steven Spitalnik, and Eldad Hod of the Department of Pathology and Cell Biology and members of the Williams Laboratory, Columbia University Fertility Center, Suh Laboratory, Clinical Microbiology Laboratory, Columbia University Laboratory of Personalized Genomic Medicine at Columbia University Medical Center and New York Presbyterian Hospital for their helpful inputs and support for this study. This study is supported by NIH grants R01HD100013 (Z.W.),